DNA

Part:BBa_K3905017

Designed by: Peter Heywood   Group: iGEM21_City_of_London_UK   (2021-10-07)
Revision as of 12:09, 7 October 2021 by Registry (Talk | contribs)

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517-5 T7p* Probe 1

Amplification probe required for the isothermal amplification of miRNA using Recombinase polymerase amplification. RPA of the miRNA required the design of the 517-5 T7p* Probe 1. Probe 1 binds to the 5' end of the miRNA. This probe is phosphorylated on its 5' end. Through phosphorylation, DNA ligase is able to ligate 517-5 T7p* Probe 1 to 517-5 T7p* Probe 1. Allowing RPA to occur for the formation of DNA. For this to occur, the forward primer 5' AACCAGCCACAAACATAATACGACTC 3' is added, which binds to this probe.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None