Coding

Part:BBa_K3726029:Design

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-06)
Revision as of 23:44, 6 October 2021 by Sbarraga (Talk | contribs)

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ppc_CDS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1099
    Illegal EcoRI site found at 1540
    Illegal PstI site found at 1684
    Illegal PstI site found at 1783
    Illegal PstI site found at 2368
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1099
    Illegal EcoRI site found at 1540
    Illegal PstI site found at 1684
    Illegal PstI site found at 1783
    Illegal PstI site found at 2368
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1099
    Illegal EcoRI site found at 1540
    Illegal BglII site found at 41
    Illegal BglII site found at 2081
    Illegal BamHI site found at 2179
    Illegal BamHI site found at 2194
    Illegal XhoI site found at 871
    Illegal XhoI site found at 1516
    Illegal XhoI site found at 1990
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1099
    Illegal EcoRI site found at 1540
    Illegal PstI site found at 1684
    Illegal PstI site found at 1783
    Illegal PstI site found at 2368
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1099
    Illegal EcoRI site found at 1540
    Illegal PstI site found at 1684
    Illegal PstI site found at 1783
    Illegal PstI site found at 2368
    Illegal NgoMIV site found at 1627
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been designed to be assembled within the part "BBa_K3726038" through Gibson.

Source

Coding sequence of this Phosphoenolpyruvate carboxylase has been found within the genome of Corynebacterium glutamicum.

Uniprot reference: https://www.uniprot.org/uniprot/P12880

References

A. Bar-Even, E. Noor, N. Lewis and R. Milo, "Design and analysis of synthetic carbon fixation pathways", 2021.