Coding

Part:BBa_K3726031:Design

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-06)
Revision as of 23:11, 6 October 2021 by Sbarraga (Talk | contribs) (→‎Design Notes)


gcl_CDS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 514
    Illegal EcoRI site found at 1630
    Illegal EcoRI site found at 1714
    Illegal PstI site found at 541
    Illegal PstI site found at 769
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 514
    Illegal EcoRI site found at 1630
    Illegal EcoRI site found at 1714
    Illegal PstI site found at 541
    Illegal PstI site found at 769
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 514
    Illegal EcoRI site found at 1630
    Illegal EcoRI site found at 1714
    Illegal BglII site found at 660
    Illegal XhoI site found at 1735
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 514
    Illegal EcoRI site found at 1630
    Illegal EcoRI site found at 1714
    Illegal PstI site found at 541
    Illegal PstI site found at 769
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 514
    Illegal EcoRI site found at 1630
    Illegal EcoRI site found at 1714
    Illegal PstI site found at 541
    Illegal PstI site found at 769
    Illegal NgoMIV site found at 231
    Illegal NgoMIV site found at 405
    Illegal NgoMIV site found at 555
    Illegal AgeI site found at 856
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been designed to be assembled within the part "BBa_K3726038" through Gibson.

Source

Coding sequence of this Glyoxylate carboligase has been obtained from Cupriavidus necator genome thought PCR. Uniprot reference:https://www.uniprot.org/uniprot/G0F004


References

H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021.