Plasmid
pGEM-SBPas

Part:BBa_K4047039

Designed by: An Nguyen   Group: iGEM21_MiamiU_OH   (2021-10-06)
Revision as of 18:08, 6 October 2021 by Registry (Talk | contribs)

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pGEM-SBPase (plasmid for deletion of SBPase)

Plasmid for deletion of fructose-1,6-bisphosphatase/ sedoheptulose-1,7-bisphosphatase in Synechococcus elongatus PCC 7942; contains all the components of pXWK3-glgC in addition to our gene

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1998
    Illegal PstI site found at 2591
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1998
    Illegal PstI site found at 2591
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1489
    Illegal BglII site found at 4478
    Illegal BamHI site found at 2345
    Illegal XhoI site found at 2433
    Illegal XhoI site found at 3675
    Illegal XhoI site found at 4474
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1998
    Illegal PstI site found at 2591
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1998
    Illegal PstI site found at 2591
    Illegal AgeI site found at 1857
    Illegal AgeI site found at 4356
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2430
    Illegal BsaI.rc site found at 864


[edit]
Categories
//plasmid
Parameters
None