Plasmid_Backbone

Part:BBa_K4047024

Designed by: An Nguyen   Group: iGEM21_MiamiU_OH   (2021-10-06)
Revision as of 17:38, 6 October 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pAM2991

Backbone sequence used for restriction digest assembly of our pF, pT, and pFT plasmids. This sequence contains the promoter, coding portion, and terminator for streptomycin/spectinomycin resistance (SmR). It also contains an origin of replication for the plasmid, a lacI promoter, LacI coding sequence for a lac repressor, lac operator, and a trc promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 7968
    Illegal XbaI site found at 2
    Illegal XbaI site found at 2546
    Illegal XbaI site found at 2860
    Illegal PstI site found at 14
    Illegal PstI site found at 2558
    Illegal PstI site found at 5013
    Illegal PstI site found at 5815
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 7968
    Illegal NheI site found at 2673
    Illegal PstI site found at 14
    Illegal PstI site found at 2558
    Illegal PstI site found at 5013
    Illegal PstI site found at 5815
    Illegal NotI site found at 515
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 7968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 7968
    Illegal XbaI site found at 2
    Illegal XbaI site found at 2546
    Illegal XbaI site found at 2860
    Illegal PstI site found at 14
    Illegal PstI site found at 2558
    Illegal PstI site found at 5013
    Illegal PstI site found at 5815
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 7968
    Illegal XbaI site found at 2
    Illegal XbaI site found at 2546
    Illegal XbaI site found at 2860
    Illegal PstI site found at 14
    Illegal PstI site found at 2558
    Illegal PstI site found at 5013
    Illegal PstI site found at 5815
    Illegal NgoMIV site found at 1043
    Illegal NgoMIV site found at 3454
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 198
    Illegal BsaI site found at 2068
    Illegal BsaI.rc site found at 4839
    Illegal BsaI.rc site found at 5605
    Illegal SapI site found at 3756


[edit]
Categories
//plasmidbackbone
Parameters
None