Coding

Part:BBa_K4055518

Designed by: Li Zejun   Group: iGEM21_Yucai_SZ   (2021-08-29)
Revision as of 15:07, 6 October 2021 by Sergeiii (Talk | contribs)

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YF1

YF1 is a fully functional histidine kinase, and its biochemical activity is modulated by blue light. As a light-regulated actuator, it underpins several applications in optogenetics17, 18. In the dark YF1 is in a kinase-active state.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 79
    Illegal NgoMIV site found at 372
    Illegal NgoMIV site found at 466
    Illegal NgoMIV site found at 598
    Illegal AgeI site found at 247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 136


Reference

Sequential conformational transitions and α-helical supercoiling regulate a sensor histidine kinase (Oskar Berntsson,2017)


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