Regulatory

Part:BBa_K4055530

Designed by: Li Zejun   Group: iGEM21_Yucai_SZ   (2021-08-30)
Revision as of 14:28, 6 October 2021 by Sergeiii (Talk | contribs)


pPhIF

This part contains PhIF, which can repress pPhIF(BBa_K1725000) under promoter PfixK2. The promoter can be activated by fixJ, which along with light-sensing protein YF1 can be switched off by 470 nm blue light and on in its absence. A strong terminator is added to avoid recombination. It was characterised by using it to drive expression of GFP with two different Ribosome Binding Sites

Reference

Part:BBa_K2598016

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None