Coding

Part:BBa_K3726003:Design

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-04)
Revision as of 13:18, 5 October 2021 by Sbarraga (Talk | contribs) (→‎Design Notes)

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CDS_PhaJ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 40
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part corresponds with the codon optimized coding sequence of PhaJ enzyme. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases

Source

Coding sequence of this (R)-specific enoyl-CoA hydratase has been found within the genome of Aeromonas Caviae Uniprot reference: https://www.uniprot.org/uniprot/O32472

References