Tag
6xHis-tag

Part:BBa_K3934015

Designed by: iGEM Uppsala 2021   Group: iGEM21_Uppsala   (2021-09-30)
Revision as of 10:03, 4 October 2021 by EmelieUU21 (Talk | contribs)


6xHis-Tag for protein purification

A 6xHis-tag is a sequence consisting of 6 histidines often used a protein tag for purification. The tag binds to certain metal ions (Ni2+, Co2+) which is useful for immobilized metal ion affinity chromatography (IMAC) [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Sources:
[1] W. T. Booth et al., ‘Impact of an N-terminal Polyhistidine Tag on Protein Thermal Stability’, ACS Omega, vol. 3, no. 1, pp. 760–768, Jan. 2018, doi: 10.1021/acsomega.7b01598.

[edit]
Categories
Parameters
None