Composite

Part:BBa_K3804008

Designed by: Ichii Shirsasuna   Group: iGEM21_UTokyo   (2021-09-29)
Revision as of 06:31, 3 October 2021 by ISS1322 (Talk | contribs)

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LasI

This part expresses LasI. LasI is a protein which synthesizes 3-oxo-C12-AHL. AHL is used for the quorum sensing system in Pseudomonas aeruginosa. It binds with LasR and forms a complex(AHL-LasR). Two AHL-LasR form a dimer and activate Las promoter. LasR has some homologues such as QscR and LuxR. In our project, We used this part in order to express AHL in yeast.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 270
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None