Coding
Part:BBa_K3885008
Designed by: XIA YAO Group: iGEM21_ZJUT-China (2021-09-30)
ClpXP
ClpXP consists of two distinct proteins, a AAA+ ATPase called ClpX(BBa_K1911003) and a peptidase called ClpP(BBa_K1911002). We incorporate the ClpX and ClpP to be a one-part. In protein substrates, ClpX recognizes unstructured peptide sequences (called tags or degrons), like ssrA--a specific C-terminal degradation tag, proceeds to unfold stable tertiary structure in the protein, and then spools or translocates the unfolded polypeptide chain into a sequestered proteolytic compartment in ClpP for degradation into small peptide fragments (Fig. 1).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 387
Illegal EcoRI site found at 1449
Illegal EcoRI site found at 1794 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 387
Illegal EcoRI site found at 1449
Illegal EcoRI site found at 1794 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 387
Illegal EcoRI site found at 1449
Illegal EcoRI site found at 1794
Illegal BglII site found at 1628 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 387
Illegal EcoRI site found at 1449
Illegal EcoRI site found at 1794 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 387
Illegal EcoRI site found at 1449
Illegal EcoRI site found at 1794
Illegal AgeI site found at 1110
Illegal AgeI site found at 1848 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |