Coding

Part:BBa_K3753000:Design

Designed by: Jiaying Hu   Group: iGEM21_NJTech_China   (2021-09-18)
Revision as of 03:36, 3 October 2021 by HJY (Talk | contribs) (→‎Design Notes)


PAAS-Petunia


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 60
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 60
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding region of petunia PAAS was obtained by PCR amplification using Petunia hybrida genome as template. (Gen-Bank accession number DQ243784.1)

Source

The coding region of petunia PAAS was amplified by PCR using forward and reverse primers (5'-ATTCATATGGATACTATCAAAATCAACCC-3' and 5'-GGGATCCTTCTACGCATTCAGCATCATAG-3', respectively). (Gen-Bank accession number DQ243784.1)


References