Composite
PVDQGFP an

Part:BBa_K3882003

Designed by: Fan Jiayu   Group: iGEM21_BS_United_China   (2021-10-02)
Revision as of 02:11, 3 October 2021 by YUNQI (Talk | contribs)


E. bsuahlterminator (Part2)

Part 2 provides the function to up-regulate the expression of pvdQ to convert N-Acyl Homoserine Lactone (AHL) molecule into L-Homoserine Lactone (LHL) which is incapable of generating QS signals. T7 promoter serves as starting the whole synthetic gene. Lac operator is used for releasing LuxR. PVDQ protein functions as restraining P. aeruginosa from growing and activating GFP gene. GFP is a green fluorescence reporting gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None