Plasmid
detection

Part:BBa_K3882001:Design

Designed by: Fan Jiayu   Group: iGEM21_BS_United_China   (2021-09-07)
Revision as of 01:52, 3 October 2021 by YUNQI (Talk | contribs) (→‎Design Notes)


E. bsuahlscout-QS-bsuahlteminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part1 equipped with LuxR to sense and combine the autoinducer, N-Acyl Homoserine Lactone (AHL) in the environment. After that, LuxR-AHL complex will be activated and bind to luxr-AHL response QS element to initiate the expression of mcherry report gene. T7 promoter serves as starting the whole synthetic gene. Lac operator is used for releasing LuxR. LuxR functions as receiving AHL signal molecules, and activates mCherry red fluorescence gene. QS promoter is the place that LuxR can recruit RNA polymerase. mCherry is a red fluorescence reporting gene.

Source

111

References