Composite

Part:BBa_K3734025

Designed by: Xingjun Zhao   Group: iGEM21_CSU_CHINA   (2021-10-01)
Revision as of 03:53, 2 October 2021 by Registry (Talk | contribs)

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GIP-miR21T-GI-GAL4-4XmiR21T

GIP is a promoter that can be induced by glucose.And miR21T is miR21's target.When miR21 combined with miR21T, it will inhibit the expression of target gene.GI is a kind of light-activated protein. If you shine blue light on it, it will change its own structure and will be able to combined with LOV. GAL4 is a kind of protein that can find and combined with UAS DNA structure domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4405
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4300
    Illegal XhoI site found at 1953
    Illegal XhoI site found at 5001
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 899
    Illegal NgoMIV site found at 2455
    Illegal AgeI site found at 3682
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4920
    Illegal BsaI.rc site found at 216
    Illegal BsaI.rc site found at 3079


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Categories
Parameters
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