Plasmid

Part:BBa_K3887010

Designed by: Ran Wang   Group: iGEM21_BJU_China   (2021-10-01)
Revision as of 00:49, 2 October 2021 by Registry (Talk | contribs)

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pTarget-ΔTnaA

We use this plasmid changing its sgRNA sequence to knock down the gene TnaA in E.coli by CRISPR-Cas9

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1335
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 150
    Illegal BamHI site found at 1347
    Illegal XhoI site found at 126
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1410


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Categories
Parameters
None