Composite

Part:BBa_K3990012

Designed by: Li Wenxi   Group: iGEM21_SMS_Shenzhen   (2021-10-01)
Revision as of 16:46, 1 October 2021 by Registry (Talk | contribs)

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T7+Laccase CotA [T31I;V104A;Y141C;R146C;R198H;L206Q;L355P;L475P] Expressing Construct

We will express the enzyme LaccaseCotA in BL21(DE3) E.coli to decompose the ethylene vinyl acetate copolymer(EVA).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 917
    Illegal AgeI site found at 1224
    Illegal AgeI site found at 1380
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None