Coding

Part:BBa_K3887001:Design

Designed by: Ran Wang   Group: iGEM21_BJU_China   (2021-10-01)
Revision as of 16:12, 1 October 2021 by Registry (Talk | contribs)

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SttH


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 550
    Illegal PstI site found at 337
    Illegal PstI site found at 436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 550
    Illegal PstI site found at 337
    Illegal PstI site found at 436
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 550
    Illegal XhoI site found at 1081
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 550
    Illegal PstI site found at 337
    Illegal PstI site found at 436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 178
    Illegal EcoRI site found at 550
    Illegal PstI site found at 337
    Illegal PstI site found at 436
    Illegal NgoMIV site found at 210
    Illegal NgoMIV site found at 468
    Illegal NgoMIV site found at 1171
    Illegal NgoMIV site found at 1437
    Illegal AgeI site found at 356
    Illegal AgeI site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Bromo for indole and Tryptophan


Source

Trp 6-halogenase from Streptomyces toxytricini (SttH)


References