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Part:BBa_K3859000

Designed by: TianYi Huang   Group: iGEM21_GreatBay_SZ   (2021-10-01)
Revision as of 15:39, 1 October 2021 by Registry (Talk | contribs)

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GBSZ ARTAG barcode

BBa_K3859000 is one of our barcodes which contains specific DNA sequences. This barcode has the meaning of GBSZ ARTAG. This word includes our team name and the products we made, which are very representative. Barcode is a unique DNA sequence which we inserted into yeast spore in order to achieve an efficient, simple and durable spore detection system. We will use Cas12a to detect the barcode in spore in order to identify the authenticity of items, such as artworks. Firstly, we transferred the words GBSZ ARTAG into DNA sequence using the DNA writer website, Then we attached a cpf1-PAM sequence(TTTA)to upstream of the barcode sequences for CRISPR Cas12a detection. Finally, this short segment of DNA was inserted into yeast for spore production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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