Coding
Part:BBa_K3888005:Design
Designed by: Linhan Lei Group: iGEM21_SJTang (2021-10-01)
UppE Upstream Flanking Region
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 694
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 694
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 694
Illegal BglII site found at 333
Illegal XhoI site found at 826 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 694
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 694
Illegal NgoMIV site found at 38
Illegal NgoMIV site found at 119
Illegal NgoMIV site found at 157
Illegal NgoMIV site found at 831
Illegal NgoMIV site found at 943 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 538
Design Notes
Flanking region for 1k bp
Source
Rhodopseudomonas palustris CGA009