Coding

Part:BBa_K3814030:Design

Designed by: Simon Tang   Group: iGEM21_Sydney_Australia   (2021-10-01)
Revision as of 15:12, 1 October 2021 by Registry (Talk | contribs)

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GmR gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 502
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Where possible, restriction enzymes were removed to minimise off-target effects. Substitute bases were chosen to most closely match the natural codon frequency in bacteria.



Source

Sourced from pUCP24 (https://www.ncbi.nlm.nih.gov/nuccore/U07167.1)

References