Coding

Part:BBa_K3799004

Designed by: Shubhamay Das,Lakshmi Prakash   Group: iGEM21_IISER_Kolkata   (2021-09-30)
Revision as of 14:47, 1 October 2021 by Lekki23 (Talk | contribs)


Nisin PV, A synthetic antimicrobial peptide

This antimicrobial peptide is a synthetic variant of Nisin A produced naturally in Lactococcus lactis.Mutation of Serine to proline at position 29 (S29P) combined with an isoleucine to valine substitution at position 30 (I30V) helps in making Nisin PV resistant to nisin resistant protein which is expressed on the cell surface of certain pathogenic strains like Streptococcus agalactiae which can cleave Nisin A making it less active.Nisin Pv is not only resistant to NRP but also shows enhanced antimicrobial activity against S.uberis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None