Coding

Part:BBa_K3799000

Designed by: Shubhamay Das   Group: iGEM21_IISER_Kolkata   (2021-09-30)
Revision as of 14:40, 1 October 2021 by Lekki23 (Talk | contribs)


EiCsm6,A CRISPR system endoribonuclease

This part encodes Csm6, a type III-A CRISPR-associated protein that degrades cyclic hexa-adenylate activator using both CARF and HEPN domains from Enterococcus italicus.cyclic hexa-AMP recognition by the CARF domain activates the Csm6 HEPN domains for collateral RNA degradation.It protects bacteria against transformation with plasmids containing DNA homologous to its spacer regions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 314
    Illegal XhoI site found at 367
    Illegal XhoI site found at 1054
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part
//cds
//function/crispr
Parameters
chassisE. Coli