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Part:BBa_K3814017:Design

Designed by: Simon Tang   Group: iGEM21_Sydney_Australia   (2021-10-01)
Revision as of 13:50, 1 October 2021 by Registry (Talk | contribs)

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5' Homology Arm for Cluster 4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 5
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 5
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Self-dimerisation and hairpin formation metrics were calculated on PrimerSelect. Lowest values are as follows:

Self-dimerisation: -1.6kc/m (worst possible is -83.9kc/m) Hairpin formation: 1.2kc/m



Source

iGEM USYD 2021

References