Other

Part:BBa_K3814017

Designed by: Simon Tang   Group: iGEM21_Sydney_Australia   (2021-10-01)
Revision as of 13:50, 1 October 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


5' Homology Arm for Cluster 4

This 50bp section of DNA is going to be used as a homology arm to insert the next gene cluster into. These bases have been designed to minimise self-dimerisation and hairpin formations, as it will also exist as a separate primer.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 5
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 5
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None