Coding

Part:BBa_K415202

Designed by: Grant Robinson   Group: iGEM10_MIT   (2010-08-20)
Revision as of 10:34, 1 October 2021 by Igkoni (Talk | contribs) (→‎Thessaly 2021 Literature Characterization)


cymR Repressor Protein

The cymR repressor tightly and efficiently binds the cmt operator. In conjunction with part BBa_K415200, this repressor may be used to create an inverter or similar logical circuitry. cymR changes conformation upon induction with cumate (isopropylbenozate), and may be used in a manner similar to the lacI/IPTG system.

The cymR repressor appears to bind the complementary cmt operator more efficiently than lac repressor binds the lac operator, according to Choi, et al. in a recent (as of 2010) paper titled, "A novel, versatile, and tightly regulated expression system for E. Coli strains."

Thessaly 2021 Literature Characterization

Thessaly 2021contributed to the characterization of this part by adding new documentation learned from the literature as to why CymR is a better repressor for the cumate kill-switch.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 357
    Illegal XhoI site found at 138
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 40
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Categories
Parameters
None