DNA

Part:BBa_K4103978

Designed by: Almiro Pires da Silva Neto   Group: iGEM21_Open_Science_Global   (2021-10-20)
Revision as of 10:20, 1 October 2021 by Registry (Talk | contribs)

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pHT43_PfuSso7d

Pfu Polymerase coding sequence optimized for use in Bacillus subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3028
    Illegal XbaI site found at 3037
    Illegal SpeI site found at 8672
    Illegal PstI site found at 8681
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3028
    Illegal NheI site found at 8514
    Illegal SpeI site found at 8672
    Illegal PstI site found at 8681
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3028
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3028
    Illegal XbaI site found at 3037
    Illegal SpeI site found at 8672
    Illegal PstI site found at 8681
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3028
    Illegal XbaI site found at 3037
    Illegal SpeI site found at 8672
    Illegal PstI site found at 8681
    Illegal AgeI site found at 7630
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2076


[edit]
Categories
Parameters
None