Plasmid
Part:BBa_K3963001
Designed by: Silja Malkewitz Group: iGEM21_Heidelberg (2021-10-01)
pBAV1k-lacI-Trc-beta-agarase YM01-3
The plasmid is combined by the Backbone from the pBWB162 plasmid (BBa_K3963000) and cloned by restriction based cloning with the insert beta-Agarase YM01-3 (BBa_K2094002). mCherry protein was cut out. The plasmid still contains the lacI behind lacIq promoter and a kanamycin selection marker.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2292
Illegal BamHI site found at 3167 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 3183
Illegal AgeI site found at 3220 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//awards/composite_part/nominee
None |