Coding

Part:BBa_K3794000:Design

Designed by: Harshraj Bumia   Group: iGEM21_KCL_UK   (2021-09-30)
Revision as of 18:54, 30 September 2021 by Harshbumia (Talk | contribs)


PVFP-5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removed signal sequence from UniProt U5Y3S6. Codon optimised for expression in E.Coli K12

Contains a 6xHis tag and TEV site for protein purification.


Source

This part was synthesised by IDT. The DNA sequence was obtained from UniProtKB: U5Y3S6, and codon optimised for expression in E.Coli

References