Composite

Part:BBa_K3739084

Designed by: Shichen Geng   Group: iGEM21_XMU-China   (2021-09-30)
Revision as of 18:44, 30 September 2021 by Registry (Talk | contribs)

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K525998(T7-RBS)-Aly01-his-hutH-FT

Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. Aly01 is a signal peptile. Direct fluorescent labeling of tetracysteine-motif-tagged secretory proteins with the biarsenical compound FlAsH is possible in situ without the need to recover the cell-free supernatant. K525998 is used for expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 275
    Illegal NgoMIV site found at 711
    Illegal NgoMIV site found at 1446
    Illegal NgoMIV site found at 1682
    Illegal AgeI site found at 111
    Illegal AgeI site found at 348
    Illegal AgeI site found at 1175
    Illegal AgeI site found at 1671
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None