Composite

Part:BBa_K3739106

Designed by: Shichen Geng   Group: iGEM21_XMU-China   (2021-09-30)
Revision as of 17:34, 30 September 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


K525998(T7-RBS)-Aly01-his-CBM-hutH-B0010

Aly01 is a signal peptide from the alginate lyase of V.natriegens, his-tag enable us to purify the linking protein, CBM can bind to cellulose and hutH is a histidine transaminase which converts histidine into urocanic acid

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 653
    Illegal NgoMIV site found at 1089
    Illegal NgoMIV site found at 1824
    Illegal NgoMIV site found at 2060
    Illegal AgeI site found at 111
    Illegal AgeI site found at 399
    Illegal AgeI site found at 489
    Illegal AgeI site found at 726
    Illegal AgeI site found at 1553
    Illegal AgeI site found at 2049
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None