Coding
NphT7

Part:BBa_K3726000

Designed by: Jorge Fernández Méndez   Group: iGEM21_MADRID_UCM   (2021-08-03)
Revision as of 15:58, 30 September 2021 by Fmjorge99 (Talk | contribs)

CDS_NphT7

This part corresponds with the protein coding sequence for an Acetoacetyl CoA synthase.

The codified enzyme (NphT7) catalyzes the irreversible condensation-decarboxylation step of 1 molecule of acetyl-coA with Malonyl-coA to transform them into Acetoacetyl-coA and CO2.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 461
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
    Illegal NgoMIV site found at 105
    Illegal NgoMIV site found at 205
    Illegal NgoMIV site found at 511
    Illegal AgeI site found at 883
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None