RNA

Part:BBa_K3718004

Designed by: Chan Pui Ching   Group: iGEM21_Hong_Kong_UCCKE   (2021-09-30)
Revision as of 14:21, 30 September 2021 by Registry (Talk | contribs)

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sgRNA for iclR with terminator - transcription template


Single guide RNA targeting iclR, for knock-out.

crRNA

Through the results returned by CHOPCHOP, our team selected the crRNA at rank no.4, which is positioned at the beginning of the iclR gene, to maximize iclR disfunctioning. The crRNA has a desirable CG content at 50% and its efficiency is predicted at 70.41%.

tracrRNA and T7 terminator

The design of tracrRNA and T7 terminator is referenced from the 2017 IGEM team iGEM17_BGIC-Union, part BBa_K2371006.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 171
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 172
    Illegal PstI site found at 186
    Illegal NotI site found at 7
    Illegal NotI site found at 179
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 172
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 172
    Illegal PstI site found at 186
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None