Composite

Part:BBa_K3804012:Design

Designed by: Ichii Shirsasuna   Group: iGEM21_UTokyo   (2021-09-29)
Revision as of 12:56, 30 September 2021 by ISS1322 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


HBD3 in yeast regulated by MET17p


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added stop codon(taa) at the end of HBD3(BBa_K3351002). Thus, the length of the sequence is 848.


Source

This part consists of MET17 promoter, kozack, MFα1tag, HBD3 and ADH1terminator.

References

TS. Moller, J. Hay, MJ. Saxton, K. Bunting, EI. Petersen, S. Kjaerulff, et al., Human beta-defensin-2 production from S. cerevisiae using the repressible MET17 promoter, Microbial cell factories 16 (1) (2017) 11, https://doi.org/10.1186/s12934-017-0627-7.