Composite

Part:BBa_K3939666

Designed by: Yu Huang   Group: iGEM21_Tianjin   (2021-09-30)
Revision as of 12:01, 30 September 2021 by Registry (Talk | contribs)

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Gal Cas9

The Cas9 proteins are regulated by Gal. Tianjin 2021 uses the Cas9 proteins to cut the chromosomes of eukaryotic cells. The highlight is that Gal regulates the expression of the Cas9 protein, which allows us to control the time of cutting yeast chromosomes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 708
    Illegal BglII site found at 1645
    Illegal BamHI site found at 2463
    Illegal XhoI site found at 4001
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3197
    Illegal AgeI site found at 85
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None