Coding

Part:BBa_K3788027

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-30)
Revision as of 09:03, 30 September 2021 by Marionisoard (Talk | contribs)

NOTOC pelB_sfGFP (without stop) _ aida-I (D33:N)

BBa_K3788026 is coding for pelB, sfGFP and AidaA. pelB is a leader sequence, it allows the protein to join the periplasm via the SEC system (unfolded proteins). Aida-I, on the other hand, is an autotransporter from the T5SS; it allow the addressing to the extracellular medium, the mutation of the 846 – 847 amino acid permit to no have protein cleavage, and attach this one to the extracellular membrane. In this contruction sfGFP permit to understand the mechanism, this protein can at the end be replaced by an other protein. sfGFP is supposed to be address to the preriplasm thanks to pelB leader and the T5SS system will address sfGFP to the extracellular membrane. Sequence was optimised for E. coli K12.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 1864
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 79


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Categories
Parameters
None