Plasmid

Part:BBa_K3731004

Designed by: Hao Yin   Group: iGEM21_Nanjing-China   (2021-09-29)
Revision as of 06:58, 30 September 2021 by Hao-Yin (Talk | contribs)


EPVM-PBBR1MCS-2

EPVM-PBBR1MCS-2 is a plasmid which can generate polyP in E.coli BL21. We inserted ppk1, vgb and mazE into the PBBR1MCS-2 vector to construct this part.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5166
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5166
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5172
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5166
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5166
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5166
    Illegal XbaI site found at 5181
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


iGEM2021_Nanjing-China Experiment

Group: Nanjing-China 2021
Author: Hao Yin

Polyphosphate kinase(PPK) polymerizes the terminal phosphate of ATP to a long chain of polyphosphate(polyP) in a freely reversible reaction.We inserted EPVM into PBBR1MCS-2 vector in order to produce polyP.

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