Signalling

Part:BBa_K3804009:Design

Designed by: iGEM21_UTokyo   Group: iGEM21_UTokyo   (2021-09-25)
Revision as of 10:24, 29 September 2021 by ISS1322 (Talk | contribs)


Reporter for quorum sensing systems in yeast with qscR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1673
    Illegal PstI site found at 2036
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1673
    Illegal PstI site found at 2036
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1673
    Illegal XhoI site found at 1595
    Illegal XhoI site found at 1646
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1673
    Illegal PstI site found at 2036
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1673
    Illegal PstI site found at 2036
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1811


Design Notes

Basically, the part is same to BBa_K1024002. When we describe the part, We used different parts which is similar to the component of BBa_K1024002 for convenience. The real sequence which we used is mutant of BBa_K3804010 which was changed LasR to QscR and pLas to pQsc.

Source

pTEF( BBa_K1024002), tVP16( BBa_K1024002), NLS( BBa_K1024002), QscR(BBa_K3804002), ADH1 terminator(BBa_J63002), pQsc(BBa_K3804003), CYC100mini( BBa_K1024002), mCherry( BBa_K1024002), CYC1 terminator(BBa_K1462070)

References