Signalling

Part:BBa_K3804010:Design

Designed by: iGEM21_UTokyo   Group: iGEM21_UTokyo   (2021-09-26)
Revision as of 09:00, 29 September 2021 by ISS1322 (Talk | contribs)


Reporter for quorum sensing systems in yeast with LasR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2567
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1580
    Illegal BamHI site found at 2738
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1928
    Illegal AgeI site found at 2125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 506
    Illegal BsaI site found at 893
    Illegal BsaI site found at 1280
    Illegal BsaI.rc site found at 547
    Illegal BsaI.rc site found at 934
    Illegal BsaI.rc site found at 1321


Design Notes

This part is based on BBa_K1024002. We deleted LuxR and pLux and added LasR, ADH1 terminator, pLas and cyc1 terminator.


Source

BBa_K1024002, LasR(BBa_C0179), pLas(BBa_K3804004), ADH1 terminator(BBa_J63002) and cyc1terminator(BBa_K1462070).

References