Coding

Part:BBa_K3788021:Design

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-28)
Revision as of 20:13, 28 September 2021 by Rpages (Talk | contribs)


Expression of GFP and of the timer device fused to the RFP protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1528
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1848
    Illegal BamHI site found at 1521
    Illegal XhoI site found at 1397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1597
    Illegal AgeI site found at 2849
    Illegal AgeI site found at 2961
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 867
    Illegal SapI.rc site found at 1351


Design Notes

-


Source

Sequence was given by LISM laboratory from CNRS, RFP and GFP coding sequence was taken corresponding to Part:BBa_E1010 and Part:BBa_E0040


References