Coding

Part:BBa_K3788020:Design

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-28)
Revision as of 20:08, 28 September 2021 by Rpages (Talk | contribs)


Expression of the timer device fused to the RFP protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 808
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1128
    Illegal BamHI site found at 801
    Illegal XhoI site found at 677
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 877
    Illegal AgeI site found at 2129
    Illegal AgeI site found at 2241
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 631


Design Notes

Source

Sequences was given by LISM laboratory from CNRS. RFP coding sequence was taken corresponding to Part:BBa_E1010


References