Coding

Part:BBa_K3788020

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-28)
Revision as of 20:08, 28 September 2021 by Rpages (Talk | contribs)


Expression of the timer device fused to the RFP protein

Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon caa and cal. caa gene is coding for Colicin A (ColA) a toxin toxin produced by E. coli strains, and active against other non-immune E.coli strains. To protect themselfs, E. coli have an imumnity protein coded by cai gene (-). cal gene is codding for a lysis protein, when this protein is produce ColA can be delivered in the extracellular medium after the E. coli cells lysis. In this contruction RFP permit to understand the mechanism, and Cal isn't able to have his lysis activity. The regulation is complex and involves many regulatory elements: terminator (+) and (-), promoters, antisense coding sequence, etc.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 808
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1128
    Illegal BamHI site found at 801
    Illegal XhoI site found at 677
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 877
    Illegal AgeI site found at 2129
    Illegal AgeI site found at 2241
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 631


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Categories
Parameters
None