Composite

Part:BBa_K3757007

Designed by: Erik Zimmer   Group: iGEM21_Tuebingen   (2021-09-28)
Revision as of 14:40, 28 September 2021 by Registry (Talk | contribs)

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35S Oak1

Gene encoding the cyclotide precursor Oak1 (BBa_K3757001), C-terminally tagged with a c-myc tag (BBa_K3757007). Regulated by the eukaryotic constitutive CaMV 35S promoter (BBa_K788000) and the 35S terminator polyadenylation signal (BBa_K1159307). In one Golden Gate cloning step, a sequence encoding a peptide can be cloned into the Oak1 precursor. This gene can then be co-expressed with CtAEP1 in Nicotiana benthamiana leading to expression of the peptide in cyclized form.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1468
    Illegal BamHI site found at 1858
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 66


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