Regulatory

Part:BBa_K3733002:Design

Designed by: Zhenhao Han   Group: iGEM21_HZAU-China   (2021-09-28)
Revision as of 10:02, 28 September 2021 by HanZhenhao (Talk | contribs) (→‎References)

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EBS-PyeaR: The promoter responsive to nitrate with extra NarL binding site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

The DNA sequence of PyeaR gets from MG1655 genome. The extra NarL binding site comes from PyeaR.

References

Squire D J P, Xu M, Cole J A, et al. Competition between NarL-dependent activation and Fis-dependent repression controls expression from the Escherichia coli yeaR and ogt promoters[J]. Biochemical Journal, 2009, 420(2): 249-257.