Composite

Part:BBa_K3789012

Designed by: MEI YING YI   Group: iGEM21_UM_Macau   (2021-09-28)
Revision as of 09:14, 28 September 2021 by Registry (Talk | contribs)

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Endochitinase enzyme secretion system

This composite part consists of TPS1 promoter, Mating factor alpha prepro-leader (MF alpha), 6 x glycine, chit42 coding sequence, and ADH1 terminator. The product of this system is a secretable endochitinase together with a HA tag. MF alpha enables the enzyme to be secreted. The 6 glycines between MF alpha and chit42 coding sequence avoid the interruption between them, enabling them to form correct spatial structure.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1023
    Illegal PstI site found at 1325
    Illegal PstI site found at 1698
    Illegal PstI site found at 1800
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1023
    Illegal PstI site found at 1325
    Illegal PstI site found at 1698
    Illegal PstI site found at 1800
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 188
    Illegal BamHI site found at 2009
    Illegal BamHI site found at 2609
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1023
    Illegal PstI site found at 1325
    Illegal PstI site found at 1698
    Illegal PstI site found at 1800
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1023
    Illegal PstI site found at 1325
    Illegal PstI site found at 1698
    Illegal PstI site found at 1800
    Illegal AgeI site found at 2027
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 406
    Illegal SapI site found at 2252
    Illegal SapI.rc site found at 1362


[edit]
Categories
Parameters
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