Coding

Part:BBa_K3788021

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-28)
Revision as of 08:01, 28 September 2021 by Registry (Talk | contribs)

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Expression of GFP and of the timer device fused to the RFP protein

Promotor induced by SOS signal. This promotor is involved in the colA regulation system of E. coli. Colicine are toxins produce by E. coli, among her the Colicine A (ColA). Cai is a immunity protein against Colicine A (COlA). ColA is toxic by inserted into the Escherichia coli inner membrane, Cai will inhibited this processus. Cal coding by cal gene cal is a polypeptide needed for the release of colicin A in the ColA system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1528
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1848
    Illegal BamHI site found at 1521
    Illegal XhoI site found at 1397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1597
    Illegal AgeI site found at 2849
    Illegal AgeI site found at 2961
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 867
    Illegal SapI.rc site found at 1351


[edit]
Categories
Parameters
None