Coding

Part:BBa_K3788020:Design

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-28)
Revision as of 07:57, 28 September 2021 by Registry (Talk | contribs)

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Expression of the timer device fused to the RFP protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 808
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1128
    Illegal BamHI site found at 801
    Illegal XhoI site found at 677
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 877
    Illegal AgeI site found at 2129
    Illegal AgeI site found at 2241
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 631


Design Notes

XbaI restriction site was delated


Source

colA sequence was given by LISM laboratory from CNRS. GFP coding sequence was taken corresponding to Part:BBa_E0040


References