Coding

Part:BBa_K3788020

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-28)
Revision as of 07:57, 28 September 2021 by Registry (Talk | contribs)

(diff) ↠Older revision | Latest revision (diff) | Newer revision → (diff)


Expression of the timer device fused to the RFP protein

Promotor induced by SOS signal. This promotor is involved in the colA regulation system of E. coli. Colicine are toxins produce by E. coli, among her the Colicine A (ColA). In this contruction GFP permit to understand the mechanism, and ColA isn't able to have his toxin activity. Cai is a immunity protein against Colicine A (COlA). ColA is toxic by inserted into the Escherichia coli inner membrane, Cai will inhibited this processus. Cal coding by cal gene cal is a polypeptide needed for the release of colicin A in the ColA system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 808
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1128
    Illegal BamHI site found at 801
    Illegal XhoI site found at 677
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 877
    Illegal AgeI site found at 2129
    Illegal AgeI site found at 2241
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 631


[edit]
Categories
Parameters
None