Coding

Part:BBa_K3788019

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-28)
Revision as of 07:52, 28 September 2021 by Registry (Talk | contribs)

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Expression of GFP and of the timer device under the control of the LexA-controlled promoter

Promotor induced by SOS signal. This promotor is involved in the colA regulation system of E. coli. Colicine are toxins produce by E. coli, among her the Colicine A (ColA). Cai is a immunity protein against Colicine A (COlA). ColA is toxic by inserted into the Escherichia coli inner membrane, Cai will inhibited this processus. Cal coding by cal gene cal is a polypeptide needed for the release of colicin A in the ColA system. In this contruction RFP permit to understand the mechanism, and Cail isn't able to have his lysis activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1528
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1848
    Illegal BamHI site found at 1521
    Illegal XhoI site found at 1397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1597
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 867
    Illegal SapI.rc site found at 1351


[edit]
Categories
Parameters
None