Coding
Part:BBa_K3788018
Designed by: Rebecca Pagès Group: iGEM21_Aix-Marseille (2021-09-28)
timer-lysis device
Cal coding by cal gene cal is a polypeptide needed for the release of colicin A in the ColA system. In this contruction RFP permit to understand the mechanism, and Cail isn't able to have his lysis activity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 585
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 905
Illegal BamHI site found at 578
Illegal XhoI site found at 454 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 654
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 408
[edit]
Categories
Parameters
None |